ScanImage® supports vDAQ for data acquisition and microscope control. vDAQ is an all-in-one controller for the most advanced laser scanning microscopes.
The 2U rackmountable vDAQ breakout shows the main functionality of the data acquisition system:
4 fast analog inputs for acquiring PMT data
12 2MHz analog outputs for microscope control (Galvos, Piezos, Pockels Cells, …)
12 1MHz analog inputs for actuator feedback
32 digital inputs/outputs for triggering / clocking
Simulated vDAQ Setup¶
For evaluation purposes, ScanImage can be configured to use a simulated vDAQ by following the below instructions:
In ScanImage, open the Resource configuration window from the startup dialog or from the Main Controls window under File>Configuration.
From the Resource Configuration window, select the “+” button. Select “DAQ” from the sidebar, and select “Simulated vDAQ”. Assign an arbitrary a name and continue
The simulated vDAQ does require any further configuration. Select the button “Show vDAQ0 Pinout” to show the breakout panel of the vDAQ.
Below is an example of a pinout window that would be seen after configuring a simulated pockels cell with simulated vDAQ ports. This pinout window can also be shown by clicking on the vDAQ0 widget in the sidebar.
A simulated vDAQ allows to configure an entire microscope system without any physical hardware connected to the computer.
For example, a user wanting to simulate an RMR scanner could configure a Vidrio RMR device and then follow the premium feature tutorials for Multiple Region of Interest (MROI) Imaging, Arbitrary Line Scanning, and Photostimulation
Focus Acquisitions will work with a simulated image being acquired.
with simulated devices configured with a simulated vDAQ, feedback signals are not simulated, so Waveform Optimization and other features making use of analog inputs cannot effectively be simulated.
Sample Wiring Diagrams¶
Wiring Diagrams are provided as a convenience. they can be printed out and serve as a checklist when making connections if their configuration suits the intended application. The vDAQ could be connected to devices like so:
RGG, Pockels, Shutter, Fast Focus
Thorlabs Bergamo scope