Channels Tab

Feature: Acquisitions

The Channels tab collects the controls for the scan phase, the virtual channel display and logging settings, the per-physical-channel acquisition settings, and the assignment of channels to Z depths. In earlier versions of ScanImage these controls were part of the Display tab; they now live on their own tab in the left panel.

Scan Phase Controls

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Control

Description

Auto Adjust Line Phase

Automatically detects and corrects the scan phase offset by analysing the channel viewport.

Channel Selection Dropdown

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Selects the display channel for line phase adjustment.

Manual Line Phase Adjustment

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Spinner control to optionally manually adjust the scan phase. (See Scan Phase Adjustment).

Channels

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The Channels panel configures the virtual channels that are received, displayed, and logged.

Tip

Double-click a table column header to bulk-set that column for multiple channels from a dialog. MATLAB syntax may be used in the dialog (e.g. 1:4 to set channels 1 through 4).

Column

Description

Name

The channel name. Defaults to the channel index and may be edited.

Receive

Toggles display of image data on the associated channel in the viewport.

Save

Toggles whether or not the image data associated with this channel is saved when file logging is enabled.

τ [s]

The time constant, in seconds, for exponential smoothing (rolling frame average) of the channel’s image data. Set to 0 to disable averaging.

Log τ

Toggles whether the exponential smoothing is also applied to the logged (saved) data. When off, smoothing affects only the live display.

Physical Channel Settings

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The Physical Channel Settings panel configures the analog acquisition settings for each physical input channel (e.g. AI0, AI1 …).

Column

Description

The physical analog input channel the row applies to (e.g. AI0).

Input Range

A dropdown indicating the voltage range mapping analog values to the digital range on the associated channel.

Invert

Toggles whether high digital values indicate black pixels or white pixels.

Subtract Offset

Toggles whether or not to apply the value in the Offset field.

Offset

Applies an offset to all PMT values for the associated physical channel. (See PMT Calibration)

Control

Description

Measure Offsets

Measures the channel offsets (dark value) for each physical channel. (See PMT Calibration)

Auto Measure Offsets

When enabled, the channel offsets are measured automatically at the start of each acquisition.

Channel to Z Assignment

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The Channel to Z Assignment panel maps virtual channels to Z depths or fast Z devices using a translator. This is used by multiplexed acquisition modes such as Light Beads, where each virtual channel corresponds to a different depth in the sample.

Control

Description

Translator

Selects the translator that defines how virtual channels are assigned. The table contents change to match the selected translator (e.g. a Z offset per channel, or a fast Z device per channel).

Assignment Table

Maps each virtual channel to its Z offset or fast Z device. Double-click a column header to bulk-set the values from a dialog using MATLAB syntax (e.g. 0 : 20 : 30 * 20).

Save | Load

Saves/Loads the current translator configuration to/from a JSON file using the header buttons.