Session-Session Alignment

Session to session alignment allows you to rotate and shift the sample relative coordinate system so that it is aligned to how the sample relative coordinate system was positioned and oriented on a previous session.

This allows for reuse of features using sample relative coordinates to keep track of where to scan, e.g. :ref:` Mosaic Scanning <tile manager>` and Cycle.

This is done via locating three fiducial marks in the sample on the first session, marking them A, B, and C, and saving their locations to file. On a subsequent session, you load the background and points created from the first session, markin the corresponding points (A, B, C) again, and click apply to transform the sample relative coordinates.

How the fiduciary marks are implemented are up to you. As a suggestion, right-angle corners could be engraved on a bezel. Corners give a precise location to mark between sessions.

This can be a first step towards aligning sample acquisition between sessions with motion detection methods following for pixel-precise alignment.

Note

motion estimation currently does not account for angular deviations, so too large an angular deviation between sessions may prevent use of motion detection for alignment to the sample between sessions.