Arbitrary Line Scanning Tab

Keyboard Shortcuts

Element	Description
Enable Arbitrary Line Scanning	Sets whether or not arbitrary line scanning will be used in the next acquisition
Calibrate Galvo Feedback	Calibrates Galvo feedback assuming that ramping of optical degree target angular position target gives linear ramp actual angular position. Cannot Monitor scanner feedback unless the galvo feedback has been calibrated.
Monitor Scanner Feedback	Monitors scanner feedback to display positions throughout a cycle while acquiring and to save to a CSV file if a TIFF is concurrently being acquired. Learn more here Verifying the Scan Path

Element

Description

Table Column	Description
name	Name of the ROI
	Stimulus function of the ROI
Time [ms]	Duration of the stimulus function in milliseconds
Power [%]	Power that the beam modulator paired with the stim scanner will be set to while executing this stim function Note This will override what is set from the beams slider in the `Acquisition Controls` panel
Enable	Whether or not the given ROI will be used to determine the scan path in the next acquisition
Display	Whether or not the ROIs data will be displayed in the viewport when acquiring.

Element	Description
Stim Function	Decides the path that the X, Y, and optionally fastZ scanners will trace for the selected ROI
Beam Function	Decides the variation of the power thoughout the execution of the selected ROI
Duration [ms]	Duration of the stim function
Repetitions	The number of times that the selected stim function should be repeated before moving to the next stim ROI in the ROI group Note If the stim ROI does not end where it starts, then the actuators will need to perform a step to get from end to start which can affect the scanners’ ability to trace exactly what was drawn.
Z Span [µm]	The size of the ROI in the z dimension for particular Stim functions that make use of it. At the time of writing, such stim functions making use of this are: - zcoil - zspiral

Positioning parameters are pulled into a table:

Element	Description
Z	Decides the path that the X, Y, and optionally fastZ scanners will trace for the selected ROI
Center X	Decides the variation of the power thoughout the execution of the selected ROI
Center Y	Duration of the stim function
Width	The number of times that the selected stim function should be repeated before moving to the next stim ROI in the ROI group
Height	The size of the ROI in the z dimension for particular Stim functions that make use of it. At the time of writing, such stim functions making use of this are: - zcoil - zspiral
Rotation [°]	Rotation of the selected ROI

Each stimulus function comes with its own optional parameters. These are listed in another table.

Beam modulator

Column	Description
Beam Modulator	[Non-Editable, set from resource configuration] Beam modulator device that has been paired with the imaging scanner.
Power	Power of the selected stim ROI

Element	Description
Style	Selects the style in which arbitrary line scan data should be displayed

This is the default display style. The fluorescence data is depicted over the parts of the path where it was collected.

There is one option for altering the display style here:

Line Width: sets the line width for the path which can help make the image formation more coherent.